Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings: A Contribution to Eliminate Some Misconceptions.
نویسندگان
چکیده
The present report shows that the effect of red and far red light on the synthesis of phenylalanine ammonia-lyase can be ascribed to the action of phytochrome Pfr. This is true not only for short term irradiation but also for the action of continuous far red light. In the latter case, the model worked out by Hartmann implicating some excited species of phytochrome has to be taken into account.
منابع مشابه
Use of Immunotitration to Demonstrate Phytochrome-Mediated Synthesis de novo of Chalcone Synthase and Phenylalanine Ammonia Lyase in Mustard Seedling Cotyledons
The suggestion [H. J. Newbury and H. Smith, Eur. J. Biochem . 117, 575 — 580 (1981)] of a posttranslational activation of accumulated enzyme precursor by phytochrome was tested in the case of phytochrome-mediated induction of chalcone synthase (EC 2.3.1.74) in mustard seedlings. The approach of immunotitration of enzyme activity was adopted since it allows to relate the amount of antigenic mate...
متن کاملPhytochrome-mediated de novo synthesis of phenylalanine ammonia-lyase: An approach using pre-induced mustard seedlings.
The molecular mechanism of enzyme (phenylalanine ammonia-lyase; EC 4.3.1.5) formation mediated by phytochrome in mustard seedlings was investigated by labeling the enzyme with deuterium followed by high resolution CsCl density gradient analysis. A favorable relationship between induced rise of activity and turnover of this short-lived enzyme (half-life 3-4 hr) was achieved by labeling pre-irrad...
متن کاملControl of Phenylalanine Ammonia-lyase and Ascorbate Oxidase in the Mustard Seedling by Light and Hoagland’s Nutrient Solution
Hoagland’s nutrient solution (HS) * was used as a tool to investigate whether or not the phyto chrome-mediated appearance of phenylalanine ammonia-lyase (PAL) * and ascorbate oxidase (AO) * in the mustard seedling cotyledons is simply a modulation of the appearance of these enzymes in the dark (basal level). HS was applied to the mustard (Sinapis alba L.) seedling at sowing instead of distille...
متن کاملRed and far red effects on phenylalanine ammonia-lyase in raphanus and sinapis seedlings do not correlate with phytochrome spectrophotometry.
In seedlings of Raphanus sativus (radish) and Sinapis alba (mustard), irradiation for 6 hours with far red light significantly increases the extractable activity of phenylalanine ammonia-lyase by the end of the light period. A schedule of 10 minutes red light-110 minutes darkness-10 minutes red-110 minutes darkness-10 minutes red-110 minutes darkness has no effect as compared to dark controls. ...
متن کاملAbsence of pfr destruction in the modulation of phenylalanine ammonia-lyase synthesis of mustard cotyledons.
The relationship between the amount of active phytochrome (Pfr) produced by 5-minute light pulses and the rate of subsequent enzyme accumulation (phenylalanine ammonia-lyase, EC 4.3.1.5) of mustard (Sinapis alba L.) cotyledons was investigated. The response rapidly adjusts to changes of the Pfr level produced by light pulses of different wavelengths. Regardless of total phytochrome levels in th...
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عنوان ژورنال:
- Plant physiology
دوره 49 1 شماره
صفحات -
تاریخ انتشار 1972